Part:BBa_K277052

3L.3_23.B2.12

3L.3_23.B2.12 is 1015 bases long and is cloned into the pGem-T vector.

3L.3_23.B2.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B2.12 is a constituent of 3L.3_23.B2 (along with 3L.3_23.B2.01, 3L.3_23.B2.02, 3L.3_23.B2.03, 3L.3_23.B2.04, 3L.3_23.B2.05, 3L.3_23.B2.06, 3L.3_23.B2.07, 3L.3_23.B2.08, 3L.3_23.B2.09, 3L.3_23.B2.10, and 3L.3_23.B2.11.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL044C (-303..950) Subunit%2C with Yme1p%2C of the mitochondrial inner membrane i-AAA protease complex%2C which is responsible for degradation of unfolded or misfolded mitochondrial gene products%3B required for growth of cells lacking the mitochondrial genome

mutation_affecting_coding_sequence YCL044C_re_remove_MmeI (588..599) removal of MmeI

forward_primer YCL044C_tagf1v1 (42..69)

reverse_primer YCL044C_tagr1v1 (453..480)

Sequence (the first 1015 bases correspond to coordinates 37754..38768 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 752
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 752
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 752
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 752
  Illegal AgeI site found at 45
  
• 1000
  COMPATIBLE WITH RFC[1000]